cd3-efluor450 (Thermo Fisher)
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cd3-efluor450
Cd3 Efluor450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3-efluor450/pm39368477-902-60-61
Average 90 stars, based on 1 article reviews
Cd3 Efluor450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3-efluor450/pm39368477-902-60-61
Average 90 stars, based on 1 article reviews
cd3-efluor450 - by Bioz Stars,
2026-10
90/100 stars
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Staining:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Fluorescence:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Control:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Concentration Assay:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Incubation:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Recombinase Polymerase Amplification:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Activation Assay:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Quantitation Assay:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Purification:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Real-time Polymerase Chain Reaction:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Western Blot:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Activity Assay:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Expressing:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Labeling:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Derivative Assay:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Cell Culture:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Immunoprecipitation:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Marker:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Enzyme-linked Immunosorbent Assay:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Bioprocessing:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Luminex:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. Software:Article Title: Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT Article Snippet: Cells were first extracellularly stained with Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia Article Snippet: LY6C-APC (dilution 1:40, BioLegend, Cat.No. Article Title: Identifying specific functional roles for senescence across cell types. Article Snippet: Cells were then stained with fluorescence-conjugated antibodies for 30 min at 4 Article Title: NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8 + T cell Effector Function Article Snippet: Samples were then stained for viability with LIVE/DEADTM Fixable Yellow Dead Cell Stain Kit (Invitrogen), blocked with Fc-block ( Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEAL×5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEAL×b12-FEAR, bsIgG1-b12-FEAL×5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. T cells were stained for T-cell markers by incubation with Article Title: Antibodies Article Snippet: T cells were derived from human donor buffy coats (Sanquin, Amsterdam, The Netherlands) and isolated using the RosetteSep human T cell enrichment cocktail (Stemcell Technologies, France, cat. no. 15061) according to manufacturer's instructions. Article Title: Antibodies Article Snippet: Serial dilutions of bsIgG1-huCD3-H101G-FEALx5T4-207-FEAR or control antibodies (bsIgG1-huCD3-H101G-FEALxb12-FEAR, bsIgG1-b12-FEALx5T4-207-FEAR) were added (final concentration ranging from 5000 to 0.0128 ng/mL; 5-fold dilutions) and plates were incubated at 37° C. for 72 h. Next, 110 μL supernatants containing T cells were transferred to round-bottom 96-well culture plates (CellStar, cat. no. 650180) and centrifuged (300×g) at 4° C. for 3 min. |